Effect of L-cysteine, Potassium Metabisulfite, Ascorbic Acid and Citric Acid on Inhibition of Enzymatic Browning in Longan

نویسندگان

  • Nutkridta Pongsakul
  • Bundit Leelasart
  • Nuansri Rakariyatham
چکیده

The effect of different anti-browning agents on partially purified longan polyphenol oxidase (PPO) activity was investigated by spectrophotometry, using catechol as a phenolic substrate. For this purpose, L-cysteine, potassium metabisulfite, ascorbic acid, and citric acid were used to inhibit the activity of longan PPO at different concentrations (0-10 mM). Lcysteine was found to be the most potent anti-browning agent (L-cysteine > potassium metabisulfite > ascorbic acid > citric acid). Pre-incubation of PPO with some anti-browning agents increased the extent of inhibition.

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تاریخ انتشار 2006